Alternatives to the 4,40-dimethoxytrityl (DMTr) protecting group
نویسندگان
چکیده
The 9-phenyland the 9-(p-tolyl)-xanthen-9-yl groups 2a and 2b are recommended as alternatives to the 4,40-dimethoxytrityl group 1 for the protection of the 50-hydroxy functions in oligonucleotide synthesis. 2004 Elsevier Ltd. All rights reserved.
منابع مشابه
TrimerDimer: an oligonucleotide-based saturation mutagenesis approach that removes redundant and stop codons
9-fluorenylmethoxycarbonyl (Fmoc) and 4,4'-dimethoxytrityl (DMTr) are orthogonal hydroxyl protecting groups that have been used in conjunction to assemble oligonucleotide libraries whose variants contain wild-type and mutant codons randomly interspersed throughout a focused DNA region. Fmoc is labile to organic bases and stable to weak acids, whereas DMTr behaves oppositely. Based on these chem...
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